Validating Molecular Markers for Barley Leaf Rust Resistance Genes <i>Rph20</i> and <i>Rph24</i>

Improving resistance to barley leaf rust (caused by Puccinia hordei) is an important breeding objective in most growing regions worldwide. The development and subsequent utilization of high-throughput PCR-based codominant molecular markers remains effective approach select genotypes with multiple genes, permitting efficient gene deployment stewardship. genes Rph20 Rph24 confer widely adult plant (APR) rust, are common European Australian germplasm (often combination), act interactively high levels resistance. Here we report on the validation insertion-deletion (indel) based PCR that highly predictive for alleles Rph20.ai Rph24.an (both referred as Rph24).